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A recording reviewed by ABC News appears to capture President Donald Trump telling associates he wanted the then-U.S. ambassador to Ukraine Marie Yovanovitch fired while speaking at a small gathering that included Lev Parnas and Igor Fruman -- two former business associates of Trump’s personal lawyer Rudy Giuliani who have since been indicted in New York.

The recording appears to contradict statements by President Trump and support the narrative that has been offered by Parnas during broadcast interviews in recent days. Sources familiar with the recording said the recording was made during an intimate April 30, 2018, dinner at the Trump International Hotel in Washington, D.C.

Trump has said repeatedly he does not know Parnas, a Soviet-born American who has emerged as a wild card in Trump’s impeachment trial, especially in the days since Trump was impeached.

"Get rid of her!" is what the voice that appears to be President Trump’s is heard saying. "Get her out tomorrow. I don't care. Get her out tomorrow. Take her out. Okay? Do it."

On the recording, it appears the two Giuliani associates are telling President Trump that the U.S. ambassador has been bad-mouthing him, which leads directly to the apparent remarks by the President. The recording was made by Fruman, according to sources familiar with the tape.

“Every President in our history has had the right to place people who support his agenda and his policies within his Administration,” White House press secretary Stephanie Grisham said.

During the conversation, several of the participants can be heard laughing with the president. At another point, the recording appears to capture Trump praising his new choice of secretary of state, saying emphatically: “[Mike] Pompeo is the best.” But the most striking moment comes when Parnas and the president discuss the dismissal of his ambassador to Ukraine.

Parnas appears to say: "The biggest problem there, I think where we need to start is we gotta get rid of the ambassador. She's still left over from the Clinton administration," Parnas can be heard telling Trump. "She's basically walking around telling everybody 'Wait, he's gonna get impeached, just wait." (Yovanovitch actually had served in the State Department since the Reagan administration.)

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House investigators have been attempting to document – in part with text messages supplied by Parnas -- an almost year-long effort on the part of Parnas and Giuliani to get Yovanovitch removed from her post. At times, the messages made public by the House Intelligence Committee show Giuliani referencing his repeated efforts to have Yovanovitch recalled from Kyiv, a push that was initially unsuccessful.

"Boy I'm so powerful I can intimidate the entire Ukrainian government,” Giuliani messaged Parnas in May 2019. “Please don't tell anyone I can't get the crooked Ambassador fired or I did three times and she's still there.”

The identities of others participating in the recorded conversation are unclear. During an early portion of the recording where video can be seen, Donald Trump Jr. appears on the recording posing for pictures with others. Sources say they were attending a larger event happening at the hotel that night for a super PAC that supports the president.

Another clip seen on the recording, according to the sources, is of individuals entering what appears to be a suite at the Trump Hotel for the intimate dinner. The phone that was recording the Trump conversation appears to be placed down on a table with the audio still recording the conversation between the commander-in-chief and other guests, according to the sources. The image of the president does not appear on the video reviewed by ABC News.

In a recent interview with MSNBC, Parnas publicly recounted his memories of the scene at the dinner and said that Trump turned to John [DeStefano], who was his deputy chief of staff at the time, and said "Fire her," he claimed.

“We all, there was a silence in the room. He responded to him, said Mr. President, we can't do that right now because [Secretary of State Mike] Pompeo hasn't been confirmed yet, that Pompeo is not confirmed yet and we don't have -- this is when [former Secretary of State Rex] Tillerson was gone, but Pompeo was confirmed, so they go, wait until -- so several conversations he mentioned it again.“

However, Pompeo had been confirmed and privately sworn in days earlier.

A copy of the recording is now in the custody of federal prosecutors in New York's Southern District, who declined to comment to ABC News.